Journal of Medicinal Plants Biotechnology

Abstract Hyssops are herbaceous and perennial plants, belonging to the Lamiaceae family. In this experiment, callus production of two hyssop species (H. officinalis and H. angustifolius called common and local, respectively) was investigated using two culture media and different levels of plant growth regulators (PGR’s) to achieve the best culture medium and PGR combination for producing callus as a starting plant material for plant regeneration and secondary metabolite production. To do this, the seeds were cultured in MS medium and after 2 weeks shoots were placed on to the MS and B5 medium and PGR treatments (control, 0.5mg/L 2,4-D, 1mg/L 2,4-D, 0.2 mg/L NAA + 0.5mg/L 2,4-D, 0.2 mg/L NAA + 1mg/L 2,4-D) and incubated in a controlled environment. Then, the callus induction time and percentage of callus producing explants, fresh and dry weight of callus and callus color were recorded. The results showed that interaction between species, medium and PGR treatments was significant at 1% level. Meanwhile, mean comparison analyses showed that the highest amount of callus production in both evaluated hyssop species was observed in MS media supplemented with 0.5 mg/L 2,4-D and 0.2 mg/L NAA, in which the common hyssop showed calli after 6 days, while the local hyssop species started callusing after 11 days. According to this research, this PGR treatment is proposed for callus induction in both species. Given the importance of these medicinal speciesof hyssop especially the local one and probability of its extinction, these results can be used for protecting this valuable herb.
 

Abstract The genus pumpkin from the family Cucurbitaceae with oily seeds has 10 species, 5 of which are of
agricultural importance. Three species C. Pepo and C. Moschata, C. Maxima are more important. The present
study aimed to investigate genetic diversity based on ISSR markers in 30 genotypes of 3 populations of
pumpkin. For this purpose, 4 primers with simple repetitive sequences (microsatellite) were used. In this study,
72 genetic locations were ranked, of which there were 72 polymorphic locations with a polygonal percentage
of 100%. To assess the genetic similarity between the population, cluster analysis using Jaccard's similarity
was used with UPGMA method. The mean genetic distance of genotypes (by Jaccard's coefficient of similarity)
was 0.77 and the mean of content (PIC) was 0.47. The ISSR11 primers had the highest PIC (0.50). With the
regret to dendrograms obtained from cluster analysis revealed a high variation among genotypes. The highest
genetic distance was between genotypes R30, R11 and R14 and the lowest genetic distance between R24 and
R27. The results of this study showed that ISSR markers can be effectively used to study the genetic variation
of pumpkin. Among the primers used, the marker ISSR11 ((GA) 8 G) was the most suitable primer for
subsequent studies.

Abstract

Abstract