Journal of Medicinal Plants Biotechnology

Abstract Helicobacter pylori, a Gram-negative bacterium, is a major causative agent of peptic ulcers and gastrointestinal cancers. To neutralize gastric acid and ensure survival in the harsh stomach environment, H. pylori relies on urease enzyme activity, which converts urea into ammonia. Hence, urease inhibitors play a critical role in combating H. pylori and managing associated diseases, including gastritis and other gastrointestinal disorders. This study evaluated the urease-inhibitory and antioxidant activities of Cydonia oblonga Mill. (quince) fruit and seed gel extract, aiming to identify natural sources of effective urease inhibitors. The urease inhibition potential was assessed using the Berthelot method (targeting jack bean urease), while antioxidant activity was measured via the 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) free radical scavenging assay. Both extracts were prepared with 80% ethanol, and inhibitory effects were quantified through enzyme inhibition percentages and IC₅₀ values for urease, alongside DPPH radical scavenging percentages. The results revealed that the seed gel extract exhibited stronger urease inhibition (IC₅₀ = 136.6 ± 7.5 μg/mL) compared to the fruit extract (IC₅₀ = 435.3 ± 10.8 μg/mL). Antioxidant activity was higher in the fruit extract (85.23%) than in the seed gel (79.66%). These findings highlight Cydonia oblonga through its suitable antioxidant, and urease-inhibitory activity for developing safer therapeutic strategies against H. pylori infection and preventing relevant complications.

Abstract Bacterial resistance is one of the problems in the world of medicine today. Many efforts are underway to find alternative methods, one of which is to use the capacity of native plants of each region. The chamber was powdered as a homogeneous mixture and its essential oil was extracted by water distillation. Then, the components of the essential oil were identified and the amount of the components was identified using a gas chromatography device connected to a mass spectrometer. GC-Mass analysis showed that the most important effective compounds with antimicrobial activity of this plant were 2- boronanone (12.36%), camphor (6.87%), 3-carn (5.35%). The range of growth inhibition zone varied from 9 to 40 mm. Essential oil with growth inhibition zone of 40 mm had the greatest effect against Staphylococcus aureus even in comparison with clindamycin, although essential oils had no effect on Pseudomonas aeruginosa and could not inhibit its growth. In general, essential oils had a better effect on gram-positive bacteria than gram-negative bacteria.