Journal of Medicinal Plants Biotechnology

Abstract High antioxidant and minerals such as Ca, Fe, ZN and vitamins such as A, C was causing high value in Basil.
But asexual reproduction basil by seeds it has diversity and high uniformity. for this reason direct regeneration
basil by tissue culture it has production of plants with high uniformity. In this experiment nodal explants of
two variety purpule and green Basil put in MS with BAP in three levels0, 1/5, 2/5 mg/L. Qualities investigated
was contains number of regeneration, number of root, number of leaf, root length, shoot fresh weight, shoot
dry weight, leaf length, shoot length. The effect of hormone treatment in all qualities in 1% was significant.
Effect of varities in number of regeneration, fresh weight and dry weight in 1% and other qualities in 5% was
significant. In examining the interactions of the variety and concentration hormons showed that was significant
on fresh weight and dry weight in level 5%. The highest number of regeneration was obtained in purple Basil
in 1/5 mg/L BAP with average regeneration 3/17. According to results purple Basil was suitable varity for
tissue culture. Also BAP in 1/5 mg/L on all qualities had the best effect.

Abstract Purslane include valuable secondary metabolit such as dopamine, noradrinalin and omega-3. This plant is used
in different industries medication, food and hygienic. Also is used in trearment of various diseases like
diabetes, heart troubles and relieve pain. Tissue culture use to produce the same plants and free of
contamination in short time. It is a useful method that can use in eugenic and genetic engineering. In this
experiment the effect of hormone BAP on the direct regeneration of purslane has been studied. Shoot tip
explant from from sterille plants derived from germination of seeds cultured in the medium MS containing
different concentrations BAP (0, 1.5, 3, 4.5 mg/L). Percent regeneration, number stems, leave number, stem
length, leaf length, fresh weight and dry weight were investigated. Analysis of variance of data showed effect
of BAP on all traits at 1% level was significanted. The highest percentage of regeneration and other trairs was
obtained at 4.5 mg/L BAP. In the control medium, 1.5 mg/L no regeneration was seen.