Callus induction and regeneration of C. mahaleb from leaf segment
Volume 3, Issue 1, November 2017, Pages 59-66
Gholam Reza Goodarzi, Fatemeh Ahmadloo
Abstract Cerasus mahaleb L. is a member of the Rosaceae family that have been used in different industries as
pharmaceutical, packaging, dyeing, chemical, sanitary, food, and perfume. For callus induction, Leaf pieces
(0.5–1.0 cm2) produced by plantlets after surface sterilization in MS medium containing 2, 4-D at concentration
of 0, 0.25, 0.5, 1, 2, and 3 mg l-1 and BAP at concentration of 0, 0.5, and 1 mg l-1, placed in a growth chamber
under dark conditions. In the next step, the calli were transferred to culture medium supplemented with growth
regulators of BAP, TDZ, and KIN and NAA in different concentrations for regeneration and then
characteristics of callus induction (%), callus diameter (mm), callus type, and callus color was recorded. 100%
callusing with the greatest diameter and friable and globular in texture with cream to light green in color were
observed on MS medium at concentration of 2 mg l-1 2, 4-D in combination with 1 mg l-1 BAP. Calli showed
no regeneration due to brown in color and highly soft and watery in texture.