Effect of growth regulators on callus induction and regeneration of Crataegus pseudohetrophylla from leaf segment
Volume 3, Issue 1, November 2017, Pages 14-22
Fatemeh Ahmadloo, Gholam Reza Goodarzi, Azadeh Salehi
Abstract Crataegus pseudohetrophylla Pojark. of the genus Crataegus is a member of the Rosaceae family that is
important in terms of ornamental, food, medicinal, exported, shelter for wildlife and birds, soil conservation
and erosion control applications. For callus induction, Leaf pieces (0.5–1.0 cm2) produced by plantlets after
surface sterilization in MS medium containing 2, 4-D at concentrations of 0.25, 0.5, 1, 2, and 3 mg l-1 and BAP
at concentrations of 0, 0.5, and 1 mg l-1, placed in a growth chamber under dark conditions. In the next step,
the calli were transferred to culture medium supplemented with growth regulators of BAP, TDZ, NAA, and
IBA in different concentrations for regeneration and then characteristics of callus induction (%), callus
diameter (mm), callus type, and callus color were recorded. 100% callusing at concentration of 2 mg l-1 2, 4-
D in combination with 0.5 and 1 mg l-1 BAP with the greatest diameter at concentration of 2 mg l-1 2, 4-D in
combination with 1 mg l-1 BAP and hard, globular and embryonic in texture with light green to dark green in
color were observed on MS medium. 100% explants forming shoots (regeneration) was obtained using of 12
and 14 mg l-1 BAP in combination with 2 mg l-1 NAA and the highest means number of shoots per explants
was observed at concentration of 10 mg l-1 BAP in combination with 2 mg l-1 NAA.