Embryogenesis and plant indirect and direct regeneration of Lavander (Lavandula angustifolia Mill) from shoot tips
Volume 3, Issue 2, March 2018, Pages 20-28
Narjes Sharif, Abdollah Mohammadi, Khodadad Mostafavi
Abstract Lavander from Lamiaceae family is one of the most important medical and aromatic plants. The present study describes calli induction and indirect and direct regeneration optimization experiments. The experimental designs were factorial (2,4-D × KIN and BAP × NAA) for calli induction and indirect regeneration trails based on completely randomized design with four replication and factorial experiment (BAP × KIN) for direct regeneration trail based on completely randomized design with three replication. The results of the experiment showed that the most callus percentage was gained in MS medium supplemented with 0.4 mg.l-1 2,4-D and 0.4 mg.l-1 kinetin. Indirect regeneration and emergence of seedlings were only from stem explants and in MS medium containing 1.6 mg.l-1 BAP plus 1 mg.l-1 kin, in which the callus were deposited after a period of time green and after about 45 days of subculture, It was formed on shoots. In the direct regeneration study, the combination of 2 mg.l-1 BAP with 2 mg.l-1 kinetin produced the highest percentage of regeneration.