Keywords = زیست فناوری
Number of Articles: 4
A review of biotechnological researches in artichoke medicinal plant (Cynara scolymus L.)

A review of biotechnological researches in artichoke medicinal plant (Cynara scolymus L.)

Volume 8, Issue 2, January 2023

https://doi.org/10.30470/jmpb.2025.2024687.1102

ALI MOHAMMADKHANI, bahram malaki, Ali Ammarlou

Abstract Artichoke medicinal plant (Cynara scolymus L.) is a herbaceous, diploid and perennial plant. This plant is native to the Mediterranean region and belongs to the Asteracea family. It is rich

in polyphenols, flavonoids, anthocyanins, phenolic compounds, inulin, coumarins, terpenes, dietary fibre, enzymes, polysaccharides, minerals and vitamins Several studies have shown that artichokes have properties such as antioxidant, anti-inflammatory,. Due to the antioxidant and hepatoprotective effects of this plant, which contains very little fat and high levels of minerals, vitamin C, fiber, inulin, polyphenols, hydroxycinnamates and flavones. But its most effective substance can be related to polyphenols, which are mainly composed of mono and dicaffeoylquinic acids and flavonoids. The first research related to tissue culture in this plant dates back to 1973. The first reports on the secondary metabolites of this plant were on synaropecarin, which was recorded and reported for the first time in 1960 by Suchy and his colleagues.Optimization of different physical conditions such as temperature, aeration, light stirring and chemical factors such as addition of growth regulators and precursors to culture media for in vitro production of these active compounds are described. Furthermore, cultures of transformed hairy roots, induced by Agrobacterium rhizogenes infection, have been discussed as an important technique to maximize the production of secondary metabolites in vitro. In this research, a general overview of the history and the time trend of biotechnology research has been made, and the problems, results, and research findings of the world's researchers have been collected.

Optimizing MS (Murashige & Skoog) media for Ferula.gummosa

Optimizing MS (Murashige & Skoog) media for Ferula.gummosa

Volume 8, Issue 1, April 2022, Pages 99-109

https://doi.org/10.30470/jmpb.2024.2025075.1105

arman haghnazari, mohammadreza azimi, Ali Ammarlou

Abstract Ferula.gummosa is one of the important medicinal plants in Iran and world. Today this plant is endangered in nature. One of the reasons for the threat is the long dormancy of galbanum seeds and its very low germination power, along with its uncontrolled harvesting. In this research, which was conducted in a completely randomized design with 3 replications and 3 treatments at the Biotechnology Research Institute of Zanjan University of Medical Sciences, galbanum plant embryos from Fars province were seprated from seeds and cultured to find the best concentration for growth in MS media with concentrations of 1/2-1/4 and complete MS media. After 30 days, traits such as seedling length, seedling weight, and percentage of germination in each medium were measured and compared. The results showed that the growth rate of this plant in MS1/4 media was higher than the two other concentrations . According to available published sources, the results of this study are reported for the first time on the effect of culture medium concentration on germination and seedling growth indices of Ferula.gummosa plant

Callus induction of different explants in <i> Salsola tragus </i> L.

Callus induction of different explants in Salsola tragus L.

Volume 2, Issue 4, July 2017, Pages 54-60

Negar moniri rad, Bahram Maleki Zanjani, Ali Ammarellou

Abstract Native plants to desert ecosystems, have unique features to ensure their survival in harsh conditions. Choghan
(local name for Salsola tragus) is an ancient desert plant, have used for medicinal properties, and was the only
washing plant in many parts of Iran. Urban culture and supply all kinds of chemical detergents have led to the
forgetfulness of the capacities of national and patriotic detergent plant. Identify and preserve of such valuable
plants that is resistant to harsh conditions and generally are halophyte, can be exploited in therapeutic
applications, detergent plant production programs, sustainable agriculture, environmental protection and
desertification. In order to study the response of callus initiation of this plant tissues, seeds were sterilized and
planted on MS medium. After two days, explants of root, stem and leaf were cultured on MS medium with
NAA hormone with three levels: 0, 5 and 10 mg per liter. After three weeks, callus formation was evaluated.
The results showed that the best explants for good callus induction, were the explants of leaf and then stem in
16 hours of light with an intensity of 3000 lux at a concentration of 5 mg of the NAA.