The classical methods of plant genetic transformation have bottlenecks to achieve plants with interesting genotypes, while achieving remarkable success. In the process of gene transfer to plants, biomolecules must be transferred to the plant cell nucleus by passing through the tough and multi-layered plant cell wall. Nanotechnology has been widely used in medicine and life sciences, and various nanomaterials such as single walled carbon nanotubes (CNTs) have been used as non-viral carriers for the transfer of biomolecules for targeted delivery. In this study, nanocarriers were prepared after functionalization of SWCNTs and changing the surface of SWCNTs. Then pDNA containing mgfp5-ER reporter gene was loaded on the functionalized SWCNTs. In the next step, gene transfer to plant cells was carried out by the prepared SWCNTs@pDNA nanocarrier. The success of gene transfer was evaluated by the fluorescent signal of mgfp5-ER reporter gene. The results shown the preparation of a nanocarrier with a high capacity to loading pDNA on its surface and the ability of the prepared nanocarrier to pass through the cell wall and successfully transfer pDNA into the plant cell. So, this method can be considered as a relatively simple method, independent of the plant species and without the need for special laboratory equipment for gene transfer to walled plant cells. Therefore, nanobiotechnology and nanomaterials can be a window of hope for improving and increasing the efficiency of conventional methods of gene transfer to plant cells.