Document Type : Original Article
Authors
1
a-Associate Professor, Department of Pharmacognosy, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran. b. Associate Professor, Evidence-based Phytotherapy and Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran.
2
Pharm. D, Department of Medicinal Chemistry, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
3
Assistant Professor, Department of Medicinal Chemistry, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract
Helicobacter pylori, a Gram-negative bacterium, is a major causative agent of peptic ulcers and gastrointestinal cancers. To neutralize gastric acid and ensure survival in the harsh stomach environment, H. pylori relies on urease enzyme activity, which converts urea into ammonia. Hence, urease inhibitors play a critical role in combating H. pylori and managing associated diseases, including gastritis and other gastrointestinal disorders. This study evaluated the urease-inhibitory and antioxidant activities of Cydonia oblonga Mill. (quince) fruit and seed gel extract, aiming to identify natural sources of effective urease inhibitors. The urease inhibition potential was assessed using the Berthelot method (targeting jack bean urease), while antioxidant activity was measured via the 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) free radical scavenging assay. Both extracts were prepared with 80% ethanol, and inhibitory effects were quantified through enzyme inhibition percentages and IC50 values for urease, alongside DPPH radical scavenging percentages. The results revealed that the seed gel extract exhibited stronger urease inhibition (IC50 = 136.6 ± 7.5 μg/mL) compared to the fruit extract (IC50 = 435.3 ± 10.8 μg/mL). Antioxidant activity was higher in the fruit extract (85.23%) than in the seed gel (79.66%). These findings highlight Cydonia oblonga through its suitable antioxidant, and urease-inhibitory activity for developing safer therapeutic strategies against H. pylori infection and preventing relevant complications.
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