Biotechnology and plant breeding, University of zabol, Itan
Abstract
Fennel (Foeniculum vulgare Mill.) is one the oldest known medicinal plants, which is naturally and commercially grown in Iran and many parts of the world. In this study, the genetic variation of 17 Iranian fennel ecotypes were investigated by ISSR molecular marker. The ISSR markers produced a total of 51 genomic DNA segments, of which 45 bands were polymorphic. The mean polymorphism per primer, mean Polymorphic Index Content (PIC), and Marker Index (MI) were 9, 0.478 and 5.92, respectively. The dendrogram was constructed using UPGMA method and Jaccard’s similarity matrix. The ecotypes were grouped under three main groups. The 2D and 3D PCoA analysis confirmed the results of the cluster analysis. The results indicated that the studied ecotypes had meaningful genetic diversity, and the genetic grouping using ISSR markers was correlated with the geographical distribution of the genotypes. This study could improve the knowledge about the Iranian fennel genetic pool, and contribute to breeding and germplasm protection programs of medicinal plants.
Zangane,K. , Fakheri,B. , Orooji,F. , Afzalifar,A. and Makhdoomi,M. A. (2016). Study of genetic diversity in Fennel (Foeniculum vulgare) ecotypes using ISSR marker. Journal of Medicinal Plants Biotechnology, 2(4), 10-19.
MLA
Zangane,K. , , Fakheri,B. , , Orooji,F. , , Afzalifar,A. , and Makhdoomi,M. A. . "Study of genetic diversity in Fennel (Foeniculum vulgare) ecotypes using ISSR marker", Journal of Medicinal Plants Biotechnology, 2, 4, 2016, 10-19.
HARVARD
Zangane K., Fakheri B., Orooji F., Afzalifar A., Makhdoomi M. A. (2016). 'Study of genetic diversity in Fennel (Foeniculum vulgare) ecotypes using ISSR marker', Journal of Medicinal Plants Biotechnology, 2(4), pp. 10-19.
CHICAGO
K. Zangane, B. Fakheri, F. Orooji, A. Afzalifar and M. A. Makhdoomi, "Study of genetic diversity in Fennel (Foeniculum vulgare) ecotypes using ISSR marker," Journal of Medicinal Plants Biotechnology, 2 4 (2016): 10-19,
VANCOUVER
Zangane K., Fakheri B., Orooji F., Afzalifar A., Makhdoomi M. A. Study of genetic diversity in Fennel (Foeniculum vulgare) ecotypes using ISSR marker. Journal of Medicinal Plants Biotechnology, 2016; 2(4): 10-19.