Stevia (Stevia rebaudiana Bertoni) is a perennial plant that well known due to natural sweeteners Diterpenes
that contributes 4-20 percent of dry matter of the plant's leaves. Since stevia multiplication is performed by
seed, transplanting, cuttings and tissue culture, this study specifically compare the chemical compounds of the
plant in different growth conditions, including natural and tissue culture conditions using plant extracts and
essential oil. The results showed that among 55 identified chemical compounds in the extract of tissue culture,
16 compounds were common and 39 others were exclusively in tissue culture condition. Also, among total of
70 chemical compounds in the extract of natural condition, 54 compounds were exclusively observed in extract
of stevia grown in natural condition. The results of GC-MS of essential oil showed that there were eight similar
compounds among natural and tissue culture samples. Therefore, among 41 identified chemical compounds in
the stevia essence obtained from tissue culture, there were only 33 of composition exclusively observed in
tissue culture conditions. Also, among 164 compounds identified in a plant essential oil grown in a natural
conditions, 156 of them were exclusively observed in natural conditions.