Journal of Medicinal Plants Biotechnology

Genetic variation of squash pumpkins population using ISSR markers

Document Type : Original Article

Authors

tayebeh hatami 1
Seyed Kamal Kazemitabar 2
Ghaffar Kiani 3
Reza Esmaeilzadeh kenari 4

1 Master student, SANRU

2 Associate Professor, Department of Agronomy and Plant Breeding, Sari Agriculture and Natural Resources University

3 Assistant Professor, Department of Agronomy and Plant Breeding, Sari Agriculture and Natural Resources University

4 Assistant Professor, Department of Food Science and Technology, Sari Agriculture and Natural Resources University

Abstract
The genus pumpkin from the family Cucurbitaceae with oily seeds has 10 species, 5 of which are of
agricultural importance. Three species C. Pepo and C. Moschata, C. Maxima are more important. The present
study aimed to investigate genetic diversity based on ISSR markers in 30 genotypes of 3 populations of
pumpkin. For this purpose, 4 primers with simple repetitive sequences (microsatellite) were used. In this study,
72 genetic locations were ranked, of which there were 72 polymorphic locations with a polygonal percentage
of 100%. To assess the genetic similarity between the population, cluster analysis using Jaccard's similarity
was used with UPGMA method. The mean genetic distance of genotypes (by Jaccard's coefficient of similarity)
was 0.77 and the mean of content (PIC) was 0.47. The ISSR11 primers had the highest PIC (0.50). With the
regret to dendrograms obtained from cluster analysis revealed a high variation among genotypes. The highest
genetic distance was between genotypes R30, R11 and R14 and the lowest genetic distance between R24 and
R27. The results of this study showed that ISSR markers can be effectively used to study the genetic variation
of pumpkin. Among the primers used, the marker ISSR11 ((GA) 8 G) was the most suitable primer for
subsequent studies.

Keywords

squash
genetic variation
ISSR markers
Journal of Medicinal Plants Biotechnology
Volume 3, Issue 1
November 2017
Pages 23-29

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