Document Type : Original Article
Authors
1
Assistant Professor, Research Division of Natural Resources, Markazi Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Arak, I.R. Iran.
2
Assistant Professor, Forest Research Division, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO),Tehran, I.R. Iran.
Abstract
Cerasus mahaleb L. is a member of the Rosaceae family that have been used in different industries as
pharmaceutical, packaging, dyeing, chemical, sanitary, food, and perfume. For callus induction, Leaf pieces
(0.5–1.0 cm2) produced by plantlets after surface sterilization in MS medium containing 2, 4-D at concentration
of 0, 0.25, 0.5, 1, 2, and 3 mg l-1 and BAP at concentration of 0, 0.5, and 1 mg l-1, placed in a growth chamber
under dark conditions. In the next step, the calli were transferred to culture medium supplemented with growth
regulators of BAP, TDZ, and KIN and NAA in different concentrations for regeneration and then
characteristics of callus induction (%), callus diameter (mm), callus type, and callus color was recorded. 100%
callusing with the greatest diameter and friable and globular in texture with cream to light green in color were
observed on MS medium at concentration of 2 mg l-1 2, 4-D in combination with 1 mg l-1 BAP. Calli showed
no regeneration due to brown in color and highly soft and watery in texture.
Keywords
