Journal of Medicinal Plants Biotechnology

The effect of plant growth regulators on callus induction of Datura metel L.

Document Type : Original Article

Authors

Mehrdad Nourazar 1
Seyed Mehdi Razavi 2
Alireza Ghasemian 3

1 Graduate Student, Department of Biology (Plant Physiology Trend), University of Mohaghegh Ardabili, Ardabil

2 Associate Professor, Department of Biology, Faculty of Basic Sciences, University of Mohaghegh Ardabili, Ardabil

3 Assistant Professor, Department of Biology, Faculty of Basic Sciences, University of Mohaghegh Ardabili, Ardabil

Abstract
Datura metel L. is an important medicinal plant that is a part of the Solanaceae family and contains important pharmaceutical compounds, including atropine and hyoscine (scopolamine), which is part of the tropane alkaloids and has known anticholinergic properties. In recent years, callus tissue induction has been introduced as a new method for obtaining secondary metabolites. The aim of this study was to inoculate callus from the isolated leaves of Datura seedlings to obtain the best hormonal concentration for maximum callus production. Datura seeds were cultured in MS medium under sterile conditions and Datura seedlings were obtained. The leaves of the seedlings were isolated and transferred to the MS medium without hormone, but the callus did not occur. In the next step, the leaves of the seedlings were transferred to the MS medium in 9 hormonal treatments containing different concentrations of auxin 2,4-D and cytokinin kinetin (Kin). the samples with light period was 16 hours of light and 8 hours of darkness and temperatures of 25 ° C for four week. The results showed that maximum callus formation in MS medium containing two hormones with concentrations of 1 mg/L 2,4-D and 1 mg/L Kin was obtained.

Keywords

Datura metel L
callus induction
tissue culture
2
4-D
Kinetin
Journal of Medicinal Plants Biotechnology
Volume 3, Issue 2
March 2018
Pages 1-7

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