Journal of Medicinal Plants Biotechnology

Embryogenesis and plant indirect and direct regeneration of Lavander (Lavandula angustifolia Mill) from shoot tips

Document Type : Original Article

Authors

Narjes Sharif 1
Abdollah Mohammadi 2
Khodadad Mostafavi 3

1 Plant breeding M. Sc. student, Department of Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran.

2 Associated Professor, Department of Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran.

3 Associated Professor, Department of Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract
Lavander from Lamiaceae family is one of the most important medical and aromatic plants. The present study describes calli induction and indirect and direct regeneration optimization experiments. The experimental designs were factorial (2,4-D × KIN and BAP × NAA) for calli induction and indirect regeneration trails based on completely randomized design with four replication and factorial experiment (BAP × KIN) for direct regeneration trail based on completely randomized design with three replication. The results of the experiment showed that the most callus percentage was gained in MS medium supplemented with 0.4 mg.l-1 2,4-D  and 0.4 mg.l-1 kinetin. Indirect regeneration and emergence of seedlings were only from stem explants and in MS medium containing 1.6 mg.l-1 BAP plus 1 mg.l-1 kin, in which the callus were deposited after a period of time green and after about 45 days of subculture, It was formed on shoots. In the direct regeneration study, the combination of 2 mg.l-1 BAP with 2 mg.l-1 kinetin produced the highest percentage of regeneration.

Keywords

Lavandula angustifolia
Calli induction
Indirect regeneration
Direct regeneration
Journal of Medicinal Plants Biotechnology
Volume 3, Issue 2
March 2018
Pages 20-28

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