Detecting DNA Polymorphism And Genetic Diversity In Hedera L. (Araliaceae) Germplasm By RAPD markers

Esfandani Bozchaloyi, Somayeh

doi:10.30470/jmpb.2025.2054387.1125

Detecting DNA Polymorphism And Genetic Diversity In Hedera L. (Araliaceae) Germplasm By RAPD markers

Document Type : Original Article

Author

somayeh esfandani bozchaloyi

university of zanjan

https://doi.org/10.30470/jmpb.2025.2054387.1125

Abstract

Assessing the genetic diversity in the population is the prerequisite to start and develop plant breeding projects. The genus Hedera L. occupies forest understories and riparian vegetation in temperate latitudes throughout Europe, N Africa and Asia. Therefore, we collected and analyzed 35 Hedera genotype, from five provinces of Iran regions. Our aims were 1) to assess genetic diversity among some of Irainian Hedera cultivars 2) is there a correlation between species genetic and geographical distance? 3) Genetic structure of populations and taxa. We showed significant differences in quantitative morphological characters in plant species. Hedera colchica depicted unbiased expected heterozygosity (UHe) in the range of 0.19. Shannon information was high (0.39) in Hedera helix cultivars. Hedera colchica cultivars showed the lowest value, 0.23. Gene flow (Nm) was relatively low (0.29) in Hedera cultivars. The Mantel test showed correlation (r = 0.86, p=0.0001) between genetic and geographical distances. We reported high genetic diversity, which clearly shows the among some of Irainian Hedera cultivars can adapt to changing environments since high genetic diversity is linked to species adaptability. Present results highlighted the utility of RAPD markers and morphometry methods to investigate genetic diversity in Hedera cultivars.

Keywords

Gene flow

Random Amplified Polymorphic DNA (RAPD)

Hedera cultivars

isolation

morphometry

Subjects