Document Type : Original Article
Authors
1
Professor, Department of Biotechnology Engineering Shahid Bahonar University of Kerman
2
MSc. student, Department of Biotechnology Engineering, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
3
Associate Professor, Department of Food Science and Technology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
4
Assistant Professor, Department of Food Science and Technology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
10.30470/jmpb.2025.2060837.1141
Abstract
The excessive and improper use of antimicrobial agents has led to the emergence of drug-resistant strains. Therefore, It necessitates the discovery and implementation of new sources to combat microbial pathogens. This study aimed to evaluate the antibacterial effect of ethanolic extract of Ziziphus spina-christi leaves against four bacteria; Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Corynebacterium glutamicum. Initial screening of the antibacterial activity of the extract was performed using the disk diffusion method. Subsequently, the minimum inhibitory concentration (MIC) was determined using the microdilution method, and the minimum bactericidal concentration (MBC) was identified through microbial culture. According to the results, the highest antibacterial effect of the leaf extract was observed against Corynebacterium glutamicum. The microdilution test showed that the MIC for all bacteria, except Escherichia coli, was 75 mg/mL. The lowest survival rate was observed in Corynebacterium glutamicum and Staphylococcus epidermidis. The MBC for these two bacteria was determined to be 150 mg/mL, while for Escherichia coli, it was 300 mg/mL. The findings of this research demonstrated that Ziziphus leaf extract has notable antibacterial activity, especially against the studied Gram-positive bacteria, and could be considered a natural source for developing of new antibacterial drugs.
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