Document Type : Original Article
Authors
1
Ph.D graduate, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan.
2
Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan
10.30470/jmpb.2026.2085111.1171
Abstract
Taxanes are diterpenoid compounds with medicinal properties, obtained from various yew species (Taxus baccata). Among them, paclitaxel (Taxol) is the most important and is widely used in cancer chemotherapy. Due to the extremely low yield of taxol in yew plants, alternative production methods are necessary. Callus and plant cell culture present an environmentally friendly alternative for producing taxol and related taxanes. To enhance taxane production in cultured callus, various strategies such as elicitation can be employed. In this study, calli were treated with the elicitors chitosan (10, 30, and 50 mg/L) and jasmonic acid (50, 100, and 150 µM) in triplicate. The calli were maintained in B5 medium supplemented with 4 mg/L 2,4-D, 1 mg/L kinetin, 0.5 mg/L gibberellin, 10 mL/L antioxidant, 30 g/L sucrose, and 7 g/L agar, and incubated in darkness at 25°C for 21 days. The levels of five major taxanes: taxol, 10-deacetylbaccatin III, baccatin III, 10-deacetyltaxol, and cephalomannine were quantified using HPLC. The results indicated that the highest taxol and 10-deacetylbaccatin III contents were achieved with 100 µM jasmonic acid treatment. The maximum baccatin III yield was observed with 50 mg/L chitosan, while 10-deacetyltaxol production was highest with 50 µM jasmonic acid. Interestingly, the highest cephalomannine accumulation was found in the control (untreated) calli.
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