Journal of Medicinal Plants Biotechnology

Effect of benzylaminopurine (BAP) and variety on Basil regeneration

Document Type : Original Article

Authors

Fereshteh Heidargholinezhad 1
hossein moradi 2

1 MSc. student in Biotechnology and Molecular Genetics of Horticultural Products, Sari Agriculture and Natural Resources University

2 Assistant Professor, Department of Horticulture, Faculty of Crop Science, Sari Agriculture and Natural Resources University Corresponding author address: Mazandaran, Sari Agriculture and Natural Resources University, Postal Code: 578.

Abstract
High antioxidant and minerals such as Ca, Fe, ZN and vitamins such as A, C was causing high value in Basil.
But asexual reproduction basil by seeds it has diversity and high uniformity. for this reason direct regeneration
basil by tissue culture it has production of plants with high uniformity. In this experiment nodal explants of
two variety purpule and green Basil put in MS with BAP in three levels0, 1/5, 2/5 mg/L. Qualities investigated
was contains number of regeneration, number of root, number of leaf, root length, shoot fresh weight, shoot
dry weight, leaf length, shoot length. The effect of hormone treatment in all qualities in 1% was significant.
Effect of varities in number of regeneration, fresh weight and dry weight in 1% and other qualities in 5% was
significant. In examining the interactions of the variety and concentration hormons showed that was significant
on fresh weight and dry weight in level 5%. The highest number of regeneration was obtained in purple Basil
in 1/5 mg/L BAP with average regeneration 3/17. According to results purple Basil was suitable varity for
tissue culture. Also BAP in 1/5 mg/L on all qualities had the best effect.

Keywords

Basil
regeneration
Benzyl aminopurin
medicinal
Journal of Medicinal Plants Biotechnology
Volume 3, Issue 1
November 2017
Pages 30-39

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