Journal of Medicinal Plants Biotechnology

Effect of different concentrations of hormome BAP on regeneration of purslane (portulac oleracea)

Document Type : Original Article

Authors

Fereshte Heidargholinezhad 1
hossein moradi 2
Mahnaz Karimi 3
Vahid Akbarpor 4

1 MSc. student in Biotechnology and Molecular Genetics of Horticultural Products, Sari Agriculture and Natural Resources University

2 Assistant Professor, Department of Horticulture, Faculty of Crop Science, Sari Agriculture and Natural Resources University.

3 Mahnaz Karimi

4 Vahid Akbarpor

Abstract
Purslane include valuable secondary metabolit such as dopamine, noradrinalin and omega-3. This plant is used
in different industries medication, food and hygienic. Also is used in trearment of various diseases like
diabetes, heart troubles and relieve pain. Tissue culture use to produce the same plants and free of
contamination in short time. It is a useful method that can use in eugenic and genetic engineering. In this
experiment the effect of hormone BAP on the direct regeneration of purslane has been studied. Shoot tip
explant from from sterille plants derived from germination of seeds cultured in the medium MS containing
different concentrations BAP (0, 1.5, 3, 4.5 mg/L). Percent regeneration, number stems, leave number, stem
length, leaf length, fresh weight and dry weight were investigated. Analysis of variance of data showed effect
of BAP on all traits at 1% level was significanted. The highest percentage of regeneration and other trairs was
obtained at 4.5 mg/L BAP. In the control medium, 1.5 mg/L no regeneration was seen.

Keywords

purslane
Direct regeneration
Medicinal plant
Journal of Medicinal Plants Biotechnology
Volume 3, Issue 1
November 2017
Pages 53-58

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