Effects of explants and hormone concentrations on regeneration of Myrtle ( Myrtus communis )

نوع مقاله : مقاله پژوهشی


1 Assistant Professor, Department of Biotechnology, Medicinal Plants Research Institute of Modern Biotechnology, Zanjan University, Zanjan, Iran

2 Associate Professor, Department of Plant Breeding, Faculty of Agriculture, Zanjan University, Zanjan, Iran.

3 Graduate of Biotechnology, Zanjan University, Zanjan, Iran.


Medicinal herb (Myrtus communis L.) is a perennial and aromatic herb with different medicinal properties. It grows mostly in the tropical and subtropical regions of the world. This study investigated the effects of important factors including plant explants and hormones concentrations on regeneration of Myrtle. Firstly seeds of Myrtle were sterilized and germinated in the Murashic-Scug medium to use for preparation of aseptic leaf, stem and root explants. So four concentrations (0-1-2-3) mg / L of regeneration hormones including kinetin, BAP, TDZ and BAP + TDZ were used invitro. Leaf explants and concentration of 3 mg / L BAP + TDZ had the highest regeneration. Because of the low germination rate of Myrtle seeds and low repropagation of vegetative protocols in this medicinal plant, our results can be useful for its mass propagation.